Overall: 37-9 TranSouth: 11-5 (First of Five) TranSouth Tournament Champion:
Postseason Results: TranSouth Tournament: Lost Freed-Hardeman 8-3 (first round), Defeated Freed-Hardeman 4-3, Lost NAIA No. 10 Union 5-3. Region XI Tournament: Lost Lindsey Wilson 2-1 (8-innings), Lost NAIA No. 20 Martin Methodist.
 
As the 2005 Trevecca softball season approached what appeared to be a promising season, some difficult turns revealed their head and made a promising season less likely. It started with the loss of the programs winningest coach and a First-Team all-American pitcher. The coaching change limited recruiting so the Trojans opened the season with only one pitcher – senior Hollie Shane – and a new coaching staff – Ernie Reynolds, Paul DuBose, and Kip Phillips. The season opener on February 1, 2005 was a double header sweep of Quincy University with a pair of 7-6 victories, the second a nine inning victory. Little did anyone know that those two 1-run wins would begin a winning streak of historic proportions.

The season opening sweep was a springboard to a double-header sweep of Mountain State and Bethel College (Tenn.) before a spring break road trip to the Tucson Invitational Games. Trevecca found nine victories over Dakota State, Viterbo, Peru State, Park University, Wayne State, Northwestern, Judson College, Wheaton College, and Minot State and a 15-0 overall record. The Arizona performance propelled the Trojans to the No. 20 position in the first regular season NAIA Top 25 ranking.

TranSouth Athletic Conference play started with a double-header sweep of Cumberland University. After sweeping two double-headers from University of the Cumberlands and Spalding University, the Trojans resumed TranSouth play and moved their season opening winning streak to 23 games with a pair of road wins over No. 15 Martin Methodist. With two wins over Bethel (Tenn.) Trevecca would face No. 11 Union University with a 25-0 record. Trevecca won the opener over Union, but the winning streak ended at 26 games as the Bulldogs won the nightcap 4-1. The streak tied the third longest winning streak in NAIA softball history and is the best start and longest winning streak in Trevecca softball history.

The Trojans continued their winning ways with a season sweep of Brescia University and finished the regular season with a 36-5 overall record and won their the first TranSouth regular season championship in school history with a 11-5 mark. The Trojans were ranked as high as No. 12 in the nation, the highest ranking in program history, and received an invitation to the NAIA Region XI tournament. Trevecca finished the season with the best record in school history at 37-9.

The season produced many awards for the team and players. First team All-TranSouth team selections were Lindsey Howard, Ashley McCool, Brandy Miller, Hollie Shane, and Jami Toomey. Second team All-TranSouth members were Laura Redmon, Evyn Terry, and Maria Trapani. On the All-Region XI First Team members are Lindsey Howard, Brandy Miller, Ashley McCool, and Hollie Shane and on the second team was Jami Toomey. The team was well represented on the TranSouth Scholar-Athlete team by Jamie Toomey, Lindsey Howard, Jodi Reimer, Ashley McCool, Hollie Shane, and Maria Trapani. There were several players honored with weekly conference awards.

When the season was over it was the Trojans that led the entire nation in batting average, hits, and stolen bases. All in all 22 team records were broken this year. Among them were wins (37), Team Batting Average (.349), runs (288), RBI (248), and stolen bases (111).